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ATCC CRL-2407 NK92大促销ATCC NK92大促销 促销价：3500 品牌：ATCC细胞 货号：NK-92 生长状态：悬浮生长 运输方式：T25瓶或干冰 器官来源：血液系统 是否是肿瘤细胞：是 细胞形态：聚团生长 物种来源：人源 组织来源：血液 Novagen Catlog: NGT-CRL-2407 Cell name: NK-92 ATCC Number: CRL-2407 Depositors: Conkwest Inc. Biosafety Level: 2 Growth property: Suspension, multicellaggregates Morphology: Lymphoblast Species: Homo sapiens (human) Source: Cell type: natural killer cell; NKcell Disease: malignant non-Hodgkin's lymphoma Antigen Expressed: CD2 +, CD7 +, CD11a +,CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16-, CD19 -, CD20 -, CD23 -, CD34 -, HLA-DR - Ethnic origin: Caucasian Age: 50 years Gender: Male Growth medium: Alpha Minimum Essentialmedium without ribonucleosides and deoxyribo-nucleosides but with 2 mM L-glutamine and 1.5 g/Lsodium bicarbonate + 0.2 mM inositol + 0.1 mM 2-mercaptoethanol + 0.02 mM folicacid + 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovineserum Temperature: 37.0℃ Subculturing: Subcultivation Ratio: 1:2 to1:3 is recommended Subculture every 2 to 3 days Comments: NK-92 is an interleukin-2 (IL-2)dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year oldCaucasian male with rapidly progressive non-Hodgkin's lymphoma. The cell line is dependent on the presenceof recombinant Il-2 and a dose as low as 10 U/ml is sufficient to maintainproliferation; cells will die within 72 hours in the absence of IL-2. The cell line is cytotoxic to a wide rangeof malignant cells; it kills both K562 cells and Daudi cells in chromiumrelease assays. NK-92 cells (after irradiation to preventproliferation) can be used effectively for immunological ex vivo purging ofleukemia from blood without compromising hematopoietic cell function. NK-92 cells have the followingcharacteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10,CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. Species determination: STR analysis(intraspecies) Human (Unique DNA Profile) TH01: 6, 9.3 D5S818: 12 13 D13S317: 9, 12 D7S820: 10, 11 D16S539: 11, 12 CSF1PO: 11, 12 Amelogenin: X, Y vWA: 18 TPOX: 8 文献报道： 1 March 2016 | Volume 7 | Article 91 Review published: 14 March 2016 doi: 10.3389/fimmu.2016.00091 Frontiers in Immunology |www.frontiersin.org
1 November 2015 | Volume 6 | Article 578 Review published: 17 November 2015 doi: 10.3389/fimmu.2015.00578 Frontiers in Immunology |www.frontiersin.org 该细胞在各大杂志上有报道，并且美国食品药品监督管理局（FDA）和伦理委员会（IRB）已经批准使用指定细胞库提供的无病原体NK-92细胞，在德国进行的临床试验，且报道临床二期的数据较为理想。 本株NK-92细胞由我公司安诺伦生物于16年2月引进，传代清晰，已做STR基因分型鉴定报告，并为该株细胞配套专用NK92无血清培养体系，使得这株细胞培养更为容易。 官方网站：www.ebioshop.cn |