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ATCC CRL-2407 NK92大促销

ATCC NK92大促销

促销价:3500


品牌:ATCC细胞

货号:NK-92

生长状态:悬浮生长

运输方式:T25瓶或干冰

器官来源:血液系统

是否是肿瘤细胞:是

细胞形态:聚团生长

物种来源:人源

组织来源:血液

Novagen Catlog: NGT-CRL-2407

Cell name: NK-92

ATCC Number: CRL-2407

Depositors: Conkwest Inc.

Biosafety Level: 2

Growth property: Suspension, multicellaggregates

Morphology: Lymphoblast

Species: Homo sapiens (human)

Source: Cell type: natural killer cell; NKcell

Disease: malignant non-Hodgkin's lymphoma

Antigen Expressed: CD2 +, CD7 +, CD11a +,CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -,

CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16-, CD19 -, CD20 -, CD23 -, CD34 -,

HLA-DR -

Ethnic origin: Caucasian

Age: 50 years

Gender: Male

Growth medium: Alpha Minimum Essentialmedium without ribonucleosides and deoxyribo-nucleosides

but with 2 mM L-glutamine and 1.5 g/Lsodium bicarbonate + 0.2 mM inositol + 0.1 mM 2-mercaptoethanol + 0.02 mM folicacid + 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovineserum

Temperature: 37.0℃

Subculturing: Subcultivation Ratio: 1:2 to1:3 is recommended Subculture every 2 to 3 days

Comments: NK-92 is an interleukin-2 (IL-2)dependent Natural Killer Cell line derived from peripheral

blood mononuclear cells from a 50 year oldCaucasian male with rapidly progressive

non-Hodgkin's lymphoma.

The cell line is dependent on the presenceof recombinant Il-2 and a dose as low as 10 U/ml is sufficient to maintainproliferation; cells will die within 72 hours in the absence of IL-2.

The cell line is cytotoxic to a wide rangeof malignant cells; it kills both K562 cells and Daudi cells in chromiumrelease assays.

NK-92 cells (after irradiation to preventproliferation) can be used effectively for immunological ex vivo purging ofleukemia from blood without compromising hematopoietic cell function.

NK-92 cells have the followingcharacteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10,CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.

Species determination: STR analysis(intraspecies) Human (Unique DNA Profile)

TH01: 6, 9.3

D5S818: 12 13

D13S317: 9, 12

D7S820: 10, 11

D16S539: 11, 12

CSF1PO: 11, 12

Amelogenin: X, Y

vWA: 18

TPOX: 8

文献报道:

1 March 2016 | Volume 7 | Article 91

Review

published: 14 March 2016

doi: 10.3389/fimmu.2016.00091

Frontiers in Immunology |www.frontiersin.org

1 November 2015 | Volume 6 | Article 578

Review

published: 17 November 2015

doi: 10.3389/fimmu.2015.00578

Frontiers in Immunology |www.frontiersin.org


该细胞在各大杂志上有报道,并且美国食品药品监督管理局(FDA)和伦理委员会(IRB)已经批准使用指定细胞库提供的无病原体NK-92细胞,在德国进行的临床试验,且报道临床二期的数据较为理想。


本株NK-92细胞由我公司安诺伦生物于16年2月引进,传代清晰,已做STR基因分型鉴定报告,并为该株细胞配套专用NK92无血清培养体系,使得这株细胞培养更为容易。


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